Amino-nanopolystyrene exposures of oyster (<i>Crassostrea gigas</i>) embryos induced no apparent intergenerational effects

Early life stages (ELS) of numerous marine invertebrates mustcope with man-made contaminants, including plastic debris, during their pelagic phase. Among the diversity particles, nano-sized known as nanoplastics, can induce effects severe outcomes in ELS various biological models, Pacific oyster Crassostrea gigas. Here, we investigated a sub-lethal dose (0.1 µg mL−1) 50 nm polystyrene nanobeads (nano-PS) amine functions on embryos (24 h exposure) and assessed consequences larval adult performances over two generations oysters. Only few were observed. Lipid analyses revealed that first-generation (G1) exposed to nano-PS displayed relative increase cardiolipin content (+9.7%), suggesting potential modification mitochondrial functioning. G1-larvae issued from showed decreases growth (−9%) lipid storage (−20%). No effect was observed at G1 stage terms growth, ecophysiological parameters (clearance respiration rates, absorption efficiency), or reproductive outputs (gonadic development, gamete quality). Second generation (G2) larvae control significant reduction after G2 embryonic exposure (−24%) compared (as first generation), while no intergenerational detected embryos. Overall, present experimental study suggests low incidence short phenotypes along entire cycle until next generation.